The PurElute™ GX DNA Gel Extraction & Cleanup Kit is designed for the easy extraction and purification of dsDNA from solutions or agarose gel in TAE or TBE buffers. A Binding Buffer containing chaotropic salt and reinforced buffer dissolves agarose gel and facilitates the capture of DNA fragments ≥70 bp in a spin column. The column is washed and the purified DNA is eluted in 15-50 µl of Elution Buffer or water.
Benefits
- High purity DNA: Eliminate primers, primer-dimers, adapters, dNTPs, salts and DNA labels
- High recovery: >75-95% recovery depending on fragment size and elution volume
- Rapid protocol: Less than 20 minutes
- Convenient processing options: Centrifuge or vacuum
- User friendly: Simple spin column format
- Purifies almost any fragment size: 70bp - 10kb
- Binding capacity: Up to 5 µg of DNA
Downstream Applications
- Further Amplification
- Sequencing
- Restriction Digestion
- Cloning
