The gold standard in gel filtration based dye
terminator removal!
Performa® DTR utilizes a fully hydrated gel filtration
matrix that provides for a convenient and simple method of dye terminator
removal for sequencing cleanup. Performa® DTR ensures removal of BigDye®
v3.1 or other dye terminators, along with dNTPs, salts and other low molecular
weight materials from sequencing reactions. DNA primers and fragments up
to 20 bases, buffers, nucleotides labeled with biotin, isotopes and other
assorted markers are also eliminated. To minimize the potential for
interference with sequencing applications, no preservatives, salts, or buffers
are used in the preparation of Performa® DTR.
Benefits
- Long read lengths: Consistently achieve read lengths
>800 bases
- More readable sequence: Identify the bases close to
the primer and obtain excellent signal strength
- Quick 2-spin protocol: Start to finish in only 5
minutes
- Ready to use: Pre-packed with a fully hydrated gel
filtration matrix
- Flexible formats: Single, 96- and 384-well
formats
- Convenient: Spin directly into the ABI PRISM® 96-well
Optical Reaction Plate or comparable plates and load directly onto capillary
sequencers
- Effective dye terminator removal of various
chemistries: BigDye® v3.1, BigDye® v1.1, DYEnamic™ DTCS Quick
Start Kit and Methods Development Kit.
