PurElute™ IEX Plasmid Kits present a novel ion-exchange membrane for purification of superior quality endotoxin-free plasmid DNA.  Membrane porosity was optimized to provide high interior surface area accessible for DNA binding and high flow rate for easy sample processing.  This method is simpler and more efficient than conventional gravity flow columns.  PurElute™ IEX Plasmid Kits have an exceedingly high binding capacity, excellent selectivity and recovery efficiency.  Purified plasmid DNA is free of impurities that may interfere with demanding downstream applications.

See our article in the Journal of Biomolecular Techniques

Benefits

• Superior quality DNA: Suitable for mammalian cell transfections and other sensitive downstream applications
• High yield: Binding capacity greater than conventional gravity flow columns
• Quick protocol: Significantly faster than conventional methods
• Simple spin column format: No waiting for gravity flow columns
• Broad applications: Purify plasmids of all sizes, cosmids, fosmids, and BACs
• Ready to use: No pre-wetting or equilibration step
• High purity DNA: Maxiprep provides endotoxin-free DNA
• PurBlue: Visualize complete lysis to maximze DNA yield

Downstream Applications

• Enzymatic modifications
• Transfection of sensitive eukaryotic cells
• Gene therapy research
• In-vitro transcription/translation
• Plasmid-mediated gene silencing