The PurElute™ Miniprep Kit was developed for rapid isolation and purification of high-copy and low-copy plasmids as well as cosmids. The kit utilizes a modified alkaline lysis protocol and can be used in a centrifuge or a vacuum manifold in less than 20 minutes processing time. An innovative spin basket design ensures enough volume under the drip director to avoid the need for a second spin during binding. Purified DNA can be used for most molecular biology downstream applications.
Benefits
- High purity DNA: Endonuclease-free
- High yield: Up to 20 µg
- Rapid protocol: Less than 20 minutes
- Excellent DNA recovery: 85-95%
- Flexible format: Centrifuge or vacuum
- User-friendly: Simple spin-column format
- PurBlue: Visualize complete lysis to maximize DNA yield
Downstream Applications
- Restriction Digestion
- PCR
- Cloning
- Sequencing
- Ligation and Transformation
