Chromatin Immunoprecipitation sequencing (ChIP-Seq)

Chromatin immunoprecipitation (ChIP) is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. This technique gives a picture of the protein-DNA interactions involved in gene regulation or chromatin organization. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states.

Sequencing millions of ChIP-enriched DNA fragments using next generation sequencing technology enables cost-effective and precise analysis of the binding sites of transcription factors, replication and transcriptional machinery, structural proteins such as histones, as well as the impact of protein modifications on genome occupancy.

Hybridization-based methods are the most common method for genome-wide analysis of DNA-binding proteins (ChIP-chip), which combines chromatin immunoprecipitation with DNA microarrays. The major limitation of this hybridization-based method is that microarrays are restricted to a fixed number of probes, thereby introducing bias.

EdgeBio's diverse set of sequencing technologies and experience means that CHiP-Seq is available to researchers with projects of many different sizes and goals.