Frequently Asked Questions - QuickStepâ„¢2 Cartridges

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No. The gel matrix is fully hydrated in water.

Cartridges may be left out at room temperature overnight if sealed in their original foil bags. If cartridges sit at room temperature outside of the bags or for excessive periods of time the gel matrix may become dehydrated.

SOPE may be left at room temp for short periods of time. It must be kept at 4° C for long-term storage in order to retain its efficacy.

Yes. Once the cartridges have been frozen, they are irreparably damaged and will lead to purification failure.

Both the SOPE resin and the cartridges should be stored at 4° C.

The presence of mineral oil in excess amounts will interfere with the performance of QuickStep™ 2. If you use mineral oil, remove as much of it as possible prior to purification.

The initial spin should be performed for 3 minutes at 750 x g. (To enhance yield of 20 µl PCR reactions, perform the initial spin at 850 x g for 3 minutes.) The second spin should be performed at 750 x g for 2 minutes.

The crack is a normal result of the manufacturing process of the cartridge and does not affect its performance. Take care to avoid the crack when loading sample drop-wise to the center of the gel column.

Yes. SOPE is critical for the removal of primers, single-stranded DNA, enzymes and other proteins.

No. This product will not remove primer-dimers from your PCR reaction.

Expect recoveries of your purified PCR product to be 75%-85% of your unpurified PCR product.

Spin the cartridge at the correct speed for an additional minute. Even though this procedure might "save" your sample, it will not be optimal and you may need to re-run the PCR reaction.

Yes. Use deionized water to bring all reactions <20 µl up to 20 µl.

The final volume of the purified PCR product will be 2-5 µl greater than the original sample volume.

There is no known upper limit to the size of fragment that can be purified. Your recoveries will decrease, however, if you try to purify fragments smaller than 100 base pairs.

The minimum volume of PCR product that may be purified on these cartridges is 20 µl. Samples < 20 µl may be brought up to ≥ 20 µl using deionized water.

The maximum volume of PCR product that may be purified on these cartridges is 50 µl.