Cartridges may be left out at room temperature overnight if sealed in their original foil bags. If cartridges sit at room temperature outside of the bags or for excessive periods of time the gel matrix may become dehydrated.
The initial spin should be performed for 3 minutes at 750 x g. (To enhance yield of 20 µl PCR reactions, perform the initial spin at 850 x g for 3 minutes.) The second spin should be performed at 750 x g for 2 minutes.
The crack is a normal result of the manufacturing process of the cartridge and does not affect its performance. Take care to avoid the crack when loading sample drop-wise to the center of the gel column.