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No. The gel matrix is fully hydrated in water.
Plates may be left out at room temperature overnight if sealed in their original foil bags. If plates sit at room temperature outside of the bags or for excessive periods of time the gel matrix may become dehydrated.
SOPE may be left at room temp for short periods of time. It must be kept at 4° C for long-term storage in order to retain its efficacy.
Yes. Once the plates have been frozen, they are irreparably damaged and will lead to purification failure.
Both the SOPE resin and the plates should be stored at 4° C.
The presence of mineral oil in excess amounts will interfere with the performance of QuickStep™ 2. If you use mineral oil, remove as much of it as possible prior to purification.
The initial spin should be performed for 2 minutes at 850 x g. The second spin should be performed at 850 x g for 5 minutes.
Yes. SOPE is critical for the removal of primers, single-stranded DNA, enzymes and other proteins.
No. This product will not remove primer-dimers from your PCR reaction.
Expect recoveries of your purified PCR product to be 75%-85% of your unpurified PCR product.
Spin the plate at the correct speed for an additional minute. Even though this procedure might "save" your sample, it will not be optimal and you may need to re-run the PCR reaction.
The final volume of the purified PCR product will be 2-5 µl greater than the original sample volume.
There is no known upper limit to the size of fragment that can be purified. Your recoveries will decrease, however, if you try to purify fragments smaller than 100 base pairs.
The minimum volume of PCR product that may be purified on these plates is 5 µl.
The maximum volume of PCR product that may be purified on these plates is 15 µl.
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