Applications
Targeted resequencing allows scientists to capture or target only the regions of interest of the human genome and then use high-throughput sequencing to assess the genetic variation within the captured DNA fragments.
Transcriptome analysis is of growing importance in understanding how altered expression of genetic variants contributes to complex diseases such as cancer, diabetes, and heart disease. Analysis of genome-wide differential RNA expression provides researchers with greater insights into biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression.
Recently discovered families of small non-coding RNA have yield new insights for understanding gene regulation at the transcriptional and post-transcriptional level. Small non-coding RNAs are typically only 18-40 nucleotides in length, however their effect on cellular processes is profound.
Recently discovered families of small non-coding RNA have yield new insights for understanding gene regulation at the transcriptional and post-transcriptional level. Small non-coding RNAs are typically only 18-40 nucleotides in length, however their effect on cellular processes is profound.
Chromatin immunoprecipitation (ChIP) is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. This technique gives a picture of the protein-DNA interactions involved in gene regulation or chromatin organization. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states.
|
