Ion Torrent Sequencing

EdgeBio is proud to offer next generation sequencing services on the ion torrent Personal Genome Machine. Geared towards high quality, longer reads, and fast turnaround, we can quickly make your sequencing and resequencing projects a success. Specific applications include de novo viral and microbial sequencing and resequencing as well as human scale amplicon resequencing.

Highlights - Greater than 250MB of high quality sequence with the 316 - Check out our EXAMPLE Data Set (E. coli DH10B) - exactly how it will be delivered to you (username is edgecommunity, password is left blank) Quality - Average of 120 bp read length and majority of reads - Q20 or greater quality score Applications - De novo Viral and Microbial Sequencing - Human Amplicon Resquencing - RNA-Seq - 16S Metagenomics Sequencing Pricing 1 314 chip w/ alignment and variant calling- $1550 1 314 chip with de novo assembly- $1950 1 316 chip w/ alignment and variant calling- $2350 1 316 chip with de novo assembly- $2950 < 10 day turn around from sample to final delivery.

Technical Documents

- ionTorrent Overview

- ionTorrent Performance Update (from Life Technologies) - March 2011

- ionTorrent Performance Update (from Life Technologies) - April 2011

White Papers and Analysis

- Blog post on 6 runs of E. coli DH10B with associated data and analysis. Data can be downloaded from the EdgeBio SFTP Data Delivery Server. Access data.edgebio.com over port 22 using the username edgecommunity. No Password is required.