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TROUBLESHOOTING GUIDE
 

ExcelaPure 96-Well UF PCR Purification Kit

OBSERVATION
POSSIBLE CAUSE
RECOMMENDATIONS

Low DNA Recovery

 Insufficient resuspension of PCR products

•Optimize mixing conditions.

•Increase mixing time.

•If using a standard liquid handling instrument:
Optimize aspiration/dispensing of PCR products.
PCR products < 100 base pairs •ExcelaPure 96-Well UF Plates cannot purify PCR products < 100 base pairs.
PCR products dried on the membrane

•Resuspend samples immediately after filtering.
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OBSERVATION
POSSIBLE CAUSE
RECOMMENDATIONS

Sample
Contamination

 PCR products not filtered completely • Apply vacuum for 5 - 10 minutes or until wells have emptied. The membrane appear shiny.
PCR products remain on the inner wall of the well

•Transfer samples carefully to the membrane.

•Avoid droplet formation on the wall of the well, as unpurified PCR products may be co-recovered with your purified PCR products.
Insufficient primer removal

•Use optional wash step.

•Optimize PCR reaction conditions.
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OBSERVATION
POSSIBLE CAUSE
RECOMMENDATIONS
No vacuum Vacuum does not engage

•With the vacuum on, press the plate to engage the vacuum.

•Check for debris around the rubber gasket.
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