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Ion AmpliSeq Fixed Panels on HCT-15 Colon Carcinoma Cell Line

One of the more powerful and exciting applications for benchtop sequencing platforms is targeted sequencing panels.  These panels allow sequencing of targeted regions of the genome amplified by multiplexed PCR amplicon pools.  This allows for rapid, sensitive, and specific amplification of targets and rapid sequencing of samples.  These panels allow us to deliver results to customers with our standard ten business day turnaround time for benchtop sequencers.  To check the performance of these panels we tested three of the Ion Torrent AmpliSeq Ready-to-use Panels with DNA isolated from a well characterized colon cancer cell line, CCL-225 (HCT-15).  The Inherited Disease Panel was also run on Craig Venter HuRef (NS12911) DNA.

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Targeted Sequencing White Papers 1 of 5: Project Design and Enrichment Techniques

EdgeBio has prepared a series of technical white papers that cover various aspects of targeted resequencing.  These documents aim to assist researchers when choosing between the myriad targeted sequencing options available, and explain how samples are handled at EdgeBio from sample receiving to data delivery.  EdgeBio will be highlighting the white papers in a series of blog posts released this week during ASHG2012 but they are all available now on the Amplicon and Targeted Resequencing page of our website.

 

 Project Design and Enrichment Techniques (PDF)

 

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Targeted Sequencing White Papers 2 of 5: Research and Clinical Applications

This is the second of five posts released this week during ASHG2012 highlighting our targeted resequencing white papers available on our website

 

 Research and Clinical Applications (PDF)

 

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Targeted Sequencing White Papers 3 of 5: Tools of the Trade

This is the third of five posts released this week during ASHG2012 highlighting our targeted resequencing white papers available on our website

 

 Tools of the Trade (PDF)

 

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Targeted Sequencing White Papers 4 of 5: Data Delivery

This is the fourth of five posts released this week during ASHG2012 highlighting our targeted resequencing white papers available on our website

 

 Data Delivery (PDF)

 

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