Frequently Asked Questions - DNA Sample Preparation

You are here

What Clients are Saying:

Edge has excellent products and excellent service.

An elution volume of 100µl is recommended for genomic DNA isolated from cultured cells using the PurElute Bacterial Genomic Kit. Smaller elution volumes may lower yield due to insufficient resuspension.

Spheroplast Buffer must be stored at -20ºC and thawed before use. The other components of the PurElute Bacterial Genomic Kit should be stored at 4ºC. The kit is stable for up to one year under these conditions.

An elution volume of 100µl is recommended for genomic DNA isolated from cultured cells using the PurElute Bacterial Genomic Kit. Smaller elution volumes may lead to lower yield due to insufficient resuspension.

Up to 10 samples can be processed in as little as 15 minutes of hands-on time.

The expected yield of genomic DNA isolated from bacteria with the PurElute Bacterial Genomic Kit is up to 40 µg of DNA. However yields of genomic DNA will vary depending on bacterial strain, quality of the starting material, growth conditions, and the amount of material processed.

The PurElute Bacterial Genomic Kit is intended for use in the isolation of genomic DNA from 2 ml to 5 ml bacterial cultures with an O.D. of 1.0. Rich growth media such as TB (terrific broth) or 2XYT will produce more bacteria (up to 5 times), but this may not lead to greater yields or higher-quality DNA.

Make sure that you are using the right spinning conditions. Low mass of DNA loaded will result in higher percentage losses of the DNA. Increase the total amount of DNA loaded to higher than 50 ng

No. Doing so will contaminate the sample with salt and other small molecules. We recommend 50µl to achieve best results. However, 100µl load will result in small contaminations (for example, salt removal of 5M NaCl will be of 95%) that will not affect most downstream applications. To achieve better purification at 100µl volume loaded, we recommend to prespin 3 minutes at 750 x g, transfer the cartridge to a clean tube, load the sample, and then spin 2 minutes at 750 x g. If volumes are larger than 100µl, we recommend splitting the sample and use two different DNA clean up cartridges.

Check the spinning conditions and make sure that the rpm has been properly calculated. Check storage conditions and make sure that the cartridges and bag were properly closed if stored at room temperature for a short time. Cartridges should be at 4°C for long-term storage.