Frequently Asked Questions - MagDTR

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Add an extra aspiration step after each ethanol removal, aspirating as slowly as possible with the pipet offset to150μl. If this doesn’t solve the problem, then try adding a third wash step.

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Blobs or unincorporated dye terminator peaks are usually a result of incomplete removal of ethanol that may contain unincorporated dye terminators. If residual ethanol is present following the first aspiration, a second aspiration is recommended. Offset the pipet to 150μl, and aspirate as slowly as possible to remove all residual ethanol. If this step alone does not solve the problem, then try adding a third wash step to the process.

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The optimum amount of resin to use is 4 µl, and it has been our observation that this volume is optimal over a broad range of conditions.

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Shake well or vortex the resin container until the resin is in complete suspension. For all subsequent pipetting steps, use a pipet to mix the suspension prior to dispensing into the sample.

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