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Blobs or unincorporated dye terminator peaks are usually a result of incomplete removal of ethanol that may contain unincorporated dye terminators. If residual ethanol is present following the first aspiration, a second aspiration is recommended. Offset the pipet to 150μl, and aspirate as slowly as possible to remove all residual ethanol. If this step alone does not solve the problem, then try adding a third wash step to the process.