Great turn around time on my 80 exome project!
Both the SOPE resin and the cartridges should be stored at 4° C.
The presence of mineral oil in excess amounts will interfere with the performance of QuickStep2. If you use mineral oil, remove as much of it as possible prior to purification.
Yes. Use deionized water to bring all reactions <20µl up to 20µl.
There is no known upper limit to the size of fragment that can be purified. Your recoveries will decrease, however, if you try to purify fragments smaller than 100 base pairs.
Edge BioSystems201 Perry Parkway, Suite 5Gaithersburg, MD 20877Tel: 1-800-326-2685Fax: 1-301-990-0881