Keep doing what you're doing.
No. This product will not remove primer-dimers from your PCR reaction.
Expect recoveries of your purified PCR product to be 75%-99%.
Yes. Use deionized water to bring all reactions <20 µl up to 20 µl.
There is no known upper limit to the size of fragment that can be purified. Your recoveries will decrease, however, if you try to purify fragments smaller than 100 base pairs.
Edge BioSystems201 Perry Parkway, Suite 5Gaithersburg, MD 20877Tel: 1-800-326-2685Fax: 1-301-990-0881