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The expected yield of genomic DNA isolated from bacteria with the PurElute Bacterial Genomic Kit is up to 40 µg of DNA. However yields of genomic DNA will vary depending on bacterial strain, quality of the starting material, growth conditions, and the amount of material processed.
The PurElute Bacterial Genomic Kit is intended for use in the isolation of genomic DNA from 2 ml to 5 ml bacterial cultures with an O.D. of 1.0. Rich growth media such as TB (terrific broth) or 2XYT will produce more bacteria (up to 5 times), but this may not lead to greater yields or higher-quality DNA.