Frequently Asked Questions - PurElute Bacterial Genomic Kit

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An elution volume of 100µl is recommended for genomic DNA isolated from cultured cells using the PurElute Bacterial Genomic Kit. Smaller elution volumes may lower yield due to insufficient resuspension.

Spheroplast Buffer must be stored at -20ºC and thawed before use. The other components of the PurElute Bacterial Genomic Kit should be stored at 4ºC. The kit is stable for up to one year under these conditions.

An elution volume of 100µl is recommended for genomic DNA isolated from cultured cells using the PurElute Bacterial Genomic Kit. Smaller elution volumes may lead to lower yield due to insufficient resuspension.

Up to 10 samples can be processed in as little as 15 minutes of hands-on time.

The expected yield of genomic DNA isolated from bacteria with the PurElute Bacterial Genomic Kit is up to 40 µg of DNA. However yields of genomic DNA will vary depending on bacterial strain, quality of the starting material, growth conditions, and the amount of material processed.

The PurElute Bacterial Genomic Kit is intended for use in the isolation of genomic DNA from 2 ml to 5 ml bacterial cultures with an O.D. of 1.0. Rich growth media such as TB (terrific broth) or 2XYT will produce more bacteria (up to 5 times), but this may not lead to greater yields or higher-quality DNA.